Figure 3
Effect of subcutaneous administration of G-CSF (60 μg/kg; 30 μL) or vehicle (NaCl 0.9% , 30 μL). (a) on infarct volume (corrected for edema). Volumes are expressed in mm3 (mean ± SEM). *P <0.05; (b) on neutrophil infiltration in infarct area. Infiltration was quantified by counting positive cells to anti-myeloperoxidase antibody on six adjacent fields of 1 mm2 on ischemic zone. All rats underwent ischemia/reperfusion, tPA treatment, and 72 hours of reperfusion. Values are mean ± SEM. *P <0.05. Scale bar: 100 μm. G-CSF, granulocyte colony stimulating factor; SEM standard error of the mean; PMN, polymorphonuclear neutrophils.