JEV replication in human microglial cells. CHME3 cells were transfected with control mimic (CM), mimic-155 (M-155) and mimic-146a (M-146a) followed by JEV infection. Virus replication was monitored at 24 hours pi. (A) JEV NS1 expression was studied using western blotting. (B) Relative levels of JEV RNA were quantified by qPCR. Data were normalized against GAPDH RNA. (C) JEV titres in culture supernatants were determined by plaque assay. GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; JEV, Japanese encephalitis virus; pi, post-infection. *, P <0.05; **, P <0.005.