JEV-induced IFN-β mRNA expression in human microglial cells. CHME3 cells were transfected with CM, M-155, M-146a, or I-155 and mock-infected or infected with JEV 24 hours later. Total RNA was extracted 24 hours pi. and levels of various transcripts were determined by qPCR normalized against those of GAPDH. (A) Relative IFNβ mRNA expression in JEV-infected cells compared to mock-infected cells. (B) Relative abundance of various ISG transcripts in JEV-infected cells compared to mock-infected cells. (C) Relative expression of IFIT1 in M-155 and I-155 transfected cells compared to CM transfected cells following JEV infection. CM, control mimic; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; I-155, h, hours; inhibitor 155; IFN, interferon; ISG, interferon-stimulated genes; JEV, Japanese encephalitis virus; M-146a, mimic 146a; M-155, mimic 155; pi, post-infection. **, P <0.005.