Figure 7

JEV-induced CFH expression in human microglial cells. (A) CHME3 cells were mock-infected or infected with JEV. Total RNA was extracted from cells at different time points. The relative mRNA levels of CFH (left panel) and CD59, CD55, and CD46 (right panel) were determined in JEV-infected cells using qPCR in comparison to that in mock-infected cells. GAPDH was used as the normalizing control. (B) CHME3 cells were treated with either DMSO as a vehicle, NF-κB (25 μM) or PI3K inhibitor (10 μM) in DMSO for 6 hours followed by JEV infection for 24 hours. Total RNA was extracted and relative levels of CFH expression was studied by qPCR in different treatments when compared with vehicle-treated cells. (C) CHME3 was transfected with CM, M-155 or M-146a. After 24 hours of transfection cells were either mock-infected or infected with JEV for another 24 hours. Total RNA was extracted and relative levels of CFH mRNA determined in JEV-infected cells in comparison to those seen in mock-infected cells. CFH, complement factor H; CM, control mimic; DMSO, Dimethyl sulfoxide; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; h, hours; JEV, Japanese encephalitis virus; M-146a, mimic 146a; M-155, mimic 155; pi, post-infection. *, P <0.05; **, P <0.005.