PAF-induced Ca2+ responses. A: Representative trace (n = 18 cells) showing change in [Ca2+]i induced by PAF (100 nM). Following a prolonged level of SOC-mediated influx of Ca2+, the perfusion of Ca2+-free PSS abolished the response. B: Results from a separate experiment showing effects of SKF96365 (20 μM) on a PAF-induced increase in [Ca2+]i (n = 21 cells). SKF96365 application, during a sustained entry of Ca2+ through SOC, effectively reduced [Ca2+] to baseline levels.