Expression of IL-6 in PAF treated human microglia. A: RT-PCR analysis for different exposure times of microglia to PAF (applied at 100 nM). B: Effects of PAF, PAF plus SKF96365, PAF plus Ca2+-free and SKF96365 applied alone (1 h treatments). Also shown are effects of LPS and LPS plus SKF96365 (6 hr treatments). GAPDH was used as a reaction standard. C: Semi-quantitative RT-PCR for effects of the different treatments. * P < 0.05 compared with unstimulated control; # P < 0.05 compared with PAF treated microglia.