Detection of apoptotic astrocytes using immunocytochemistry. Double staining for GFAP (Left column/green)) and active caspase 3 (Right column/red). Nucleuses are stained in blue with DAPI. A) Untreated cells; B) treated with QUIN 350 nM; C) treated with QUIN 500 nM; D) treated with QUIN 1200 nM; E) treated with cycloheximide 20 μg/ml for 24 hours. Arrows point apoptotic astrocytes.