Figure 1

A: (Top) Western blot analysis detecting the presence of iNOS in 40 μg total protein extracts from PC12 cells treated for 24 hr with 10 ng/ml NGF and 10 ng/ml TNF, individually or combined (Both), in the presence of 50 nM of the receptor tyrosine kinase inhibitor K252a. Positive control (Pos) is 4 μg of total protein extracts from mouse macrophages. (Bottom) RT-PCR detecting iNOS mRNA in PC12 cells treated for 24 hr with 10 ng/ml NGF and 10 ng/ml TNF, individually or combined (Both) compared to untreated cells (Cont). Internal PCR controls lacking reverse transcriptase (RT-) were performed on each sample as shown. Results shown are representative of 3 replicate experiments.