Figure 3

A: Western blot detecting iNOS in total protein extracts from PC12 cells treated for 24 hr with 10 ng/ml NGF and 10 ng/ml TNFα, either individually or simultaneously (Both). Cells were pretreated with vehicle or 0.5 μM of the generic NOS inhibitor L-NAME. Positive control (Pos) is 4 μg of total protein extracts from mouse macrophages. Results shown are representative of 3 replicate experiments. B: Western blot detecting iNOS in total protein extracts from PC12 cells simultaneously treated with 10 ng/ml NGF and 10 ng/ml TNFα (Both), in the presence or absence of a pre-treatment with varying concentrations of the iNOS-specific inhibitor 1400 W. Results shown are representative of 4 replicate experiments.