Figure 7

Western blot detecting iNOS in 40 μg total protein extracts from PC12 cells treated for 24 hr with 10 ng/ml human TNFα, 10 ng/ml NGF, or both. Twenty-four hr before treatment, cells were transfected with either an empty vector or expression vectors for chimeric receptor proteins bearing the human TNFR1 ligand binding domains and the intracellular domain of either rat p75^NTR or TrkA NGF receptors (p75^NTR, TrkA or p75NTR+TrkA). Positive control (Pos) is 40 μg of total protein extract from wild type PC12 cells treated with both rat TNFα and NGF. Membrane was re-probed for β-actin (lower panel) to control for equal protein loading and is representative from 3 independent transfections and treatments.