NADPH oxidase stimulation by P2X7 receptor activation in the presence of glial iNOS kills co-cultured neurons. Neuronal death was assessed by propidium iodide staining (PI) and chromatin condensation of neuronal nuclei by Hoechst 33342 staining (CC) 48 hours after treatment. Neuronal death induced by BzATP following LPS/IFN-γ activation, was prevented by inhibitors of iNOS (25 μM 1400W), NADPH oxidase (1 mM apocynin) and a peroxynitrite scavenger (100 μM FeTPPS) but not by a blocker of the NMDA receptor (10 μM MK-801). Statistical differences were established using ANOVA at *p < 0.05 and ***p < 0.001, the symbol # replaces * when comparing protection against LPS/IFN-γ/BzATP induced neuronal death. Statistical significance refers to the total death (black + white parts of the bar). Data expressed is mean ± SEM, n = 3 or more.