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Table 2 Prion protein or peptide (PrP106-126) does not affect neuronal survival. Neuronal-glial cultures treated once with either prion protein (5 μg/ml) or PrP106-126 (225 μg/ml) did not induce neuronal death over a period of 7 days (assessed by Hoechst 33342 to visualise chromatin condensation (CC) or propidium iodide (PI) to stain necrotic cells). However, prion protein or PrP106-126 did stimulate the proliferation of microglia in neuronal-glial cultures over the same period of time. Statistical differences were established using ANOVA at *p < 0.05, **p < 0.01 and ***p < 0.001 and are in comparison to untreated cultures (symbol *); data expressed is mean ± SEM, n = 3 or more.

From: Activation of microglial NADPH oxidase is synergistic with glial iNOS expression in inducing neuronal death: a dual-key mechanism of inflammatory neurodegeneration

Treatment PI (%) CC (%) Microglia per field
Untreated 2.0 ± 1.6 0.6 ± 0.3 22 ± 5
Prion protein 2.9 ± 0.5 0.7 ± 0.6 53 ± 8 ***
PrP106-126 1.0 ± 0.8 0.7 ± 0.4 51 ± 7 ***