Table 2 TNF-α and MCP-1 mRNA levels are selectively elevated in the entorhinal cortex of 3xTg-AD mice prior to overt amyloid plaque pathology. Total RNA was purified from microdissected entorhinal cortex from 2, 3, and 6 month-old 3xTg-AD and non-transgenic control mice. cDNA was generated and subjected to Applied Biosystems Microfluidic Card analysis, a high-throughput quantitative RT-PCR technology that facilitates the simultaneous quantitation of 23 inflammation-related transcriptional targets. Of the panel of transcripts analyzed, only TNF-α and MCP-1 transcript levels were significantly enhanced by 6 months of age specifically within the entorhinal cortex of 3xTg-AD mice (n = 6/group). These cytokine transcripts were unchanged in the entorhinal cortex of non-transgenic mice at all time-points analyzed. *p < 0.0005 when compared to the 2 month timepoint. Proinflammatory transcript expression in 3xTg-AD and non-transgenic mice in the entorhinal cortex

3 months

6 months

3 months

6 months

Fold Change (Relative to 2 months)

Fold Change (Relative to 2 months)

Fold Change (Relative to 2 months)

Fold Change (Relative to 2 months)

0.357 +/- 0.258

0.615 +/- 0.477

0.94 +/- 0.622

4.432 +/- 9.424

5.079 +/- 4.826

10.796* +/- 3.298

1.459 +/- 1.230

2.616 +/- 5.184

0.317 +/- 0.279

0.521 +/- 0.388

0.754 +/- 0.394

0.414 +/- 0.170

0.853 +/- 0.215

0.858 +/- 0.333

1.584 +/- 0.568

0.989 +/- 0.311

1.291 +/- 0.911

1.922 +/- 1.028

1.157 +/- 0.507

1.393 +/- 1.062

5.299 +/- 4.580

14.822* +/- 5.618

1.215 +/- 1.793

1.702 +/- 1.916

1.149 +/- 0.181

1.092 +/- 0.527

1.054 +/- 0.178

0.922 +/- 0.312

1.292 +/- 0.830

1.900 +/- 2.225

0.811 +/- 0.218

1.459 +/- 0.740

2.216 +/- 2.921

4.900 +/- 5.817

1.632 +/- 1.604

3.340 +/- 4.726

0.110 +/- 0.075

0.190 +/- 0.180

0.350 +/- 0.234

0.424 +/- 0.418

0.932 +/- 0.243

1.371 +/- 0.687

0.788 +/- 0.290

0.568 +/- 0.285

0.413 +/- 0.478

0.899 +/- 0.925

1.528 +/- 1.502

0.893 +/- 0.859

0.402 +/- 0.182

0.444 +/- 0.346

15.159 +/- 22.054

13.181 +/- 13.192

1.000 +/- 0.317

1.215 +/- 0.567

1.029 +/- 0.076

0.733 +/- 0.202

1.031 +/- 0.067

0.969 +/- 0.399

0.890 +/- 0.137

0.690 +/- 0.337

3.149 +/- 2.363

2.191 +/- 1.772

0.549 +/- 0.488

0.605 +/- 0.440

0.876 +/- 0.603

2.190 +/- 1.978

0.329 +/- 0.433

9.954 +/- 17.454

0.888 +/- 0.158

0.812 +/- 0.460

0.822 +/- 0.473

0.714 +/- 0.451

0.839 +/- 0.237

0.843 +/- 0.394

1.002 +/- 0.207

0.628 +/- 0.237

1.066 +/- 0.225

1.270 +/- 0.609

1.165 +/- 0.232

0.966 +/- 0.323

0.725 +/- 0.265

0.538 +/- 0.261

2.105 +/- 1.357

1.369 +/- 0.485

0.955 +/- 0.171

0.900 +/- 0.424

0.884 +/- 0.173

0.741 +/- 0.366

0.887 +/- 0.214

0.811 +/- 0.266

0.959 +/- 0.229

0.974 +/- 0.618