Nissl stained sections demonstrating the overall morphological integrity of the P7 hippocampus slices cultured in serum-free media. (A) Control slice cultured for 5 DIV. (B) LPS (5 μg/ml/24 h) treated slice at 5 DIV. (C) LPS (5 μg/ml/24 h) and TSA (20 nM/24 h) treated slice at 14 DIV. The neuronal layers could be recognized despite the different treatments. DG = dentate gyrus, CA3 = cornu ammonis 3 and CA1 = cornu ammonis 1. Scale bars equal 200 μm.