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Figure 9 | Journal of Neuroinflammation

Figure 9

From: A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices

Figure 9

Astrocytes in P7 hippocampus slice stained with a GFAP antibody. (A) Immediately after sectioning, astrocytes displayed a star-shaped phenotype (arrows), also the "end-feet" enveloping microvessels were clearly visible (dashed arrows). (B) After 2 DIV the GFAP-positive cells started to transform into fibrous cells with long processes and (C) by 5 DIV, astrocytes with long processes covered the whole slice, with the semblance of an astrocytic scar. Scale bars in A and B equal 50 μm, C = 100 μm.

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