Figure 9From: A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slicesAstrocytes in P7 hippocampus slice stained with a GFAP antibody. (A) Immediately after sectioning, astrocytes displayed a star-shaped phenotype (arrows), also the "end-feet" enveloping microvessels were clearly visible (dashed arrows). (B) After 2 DIV the GFAP-positive cells started to transform into fibrous cells with long processes and (C) by 5 DIV, astrocytes with long processes covered the whole slice, with the semblance of an astrocytic scar. Scale bars in A and B equal 50 μm, C = 100 μm.Back to article page