Figure 5

Immunization with oligomeric Aβ at 12–16 months decreases microglia reactivity to a greater extent than immunization with fAβ. Photomicrographs of immunohistochemical staining of microglial reactivity (MAC-1, brown) in Tg2576 untreated or immunized with CFA, Oligo Aβ and fAβ at 12–16 mos (A). Scale bar = 50 microns Image analysis of MAC-1 (B) and CD45 (C) immunoreactivity in hippocampus and cortex of animals immunized at 12–16 months. Values for each animal were the average of 6–8 images per section which resulted in analysis of most to all of the area of the section. Bars represent group mean ± SD of n mice per group: Control n = 7, CFA n = 4, oligo Aβ n = 6–7, fAβ n = 5. By ANOVA, for MAC, CFA: oligo Aβ *p < 0.01; for CD45, CFA: oligo Aβ *p < 0.001. Data for each marker are from one assay representative of 2 independent assays.