Figure 5

HIV-1 tat injection activates SVMs and incites IMG. (A) Ependymal loss (top left) and subventricular microgliosis (bottom left) 24 h following injection of 2.0 nmol tat protein but not in animals injected with deactivated tat (right panels). (B) To determine if tat-activated SVMs migrated in situ we rendered timelapse confocal movies 24 h post-injection. Colored arrowheads demonstrate three SVMs which migrate from the region near the ventricle (green line) deep into the parenchyma (colored dashed lines). Field measures ~200 μm horizontally. See also Video 5.