Figure 3

BW-B 70C inhibits expression of iNOS and p65 in vivo. (A) Photomicrographs of immunohistochemistry of rat brain sections at 24 h reperfusion after 20 min MCAO showing remarkable iNOS expression in vehicle-treated (ii) but not in BW-B 70C-treated rats (iii). (B) BW-B 70C inhibited IR-induced iNOS gene expression measured as mRNA levels at 3 h of reperfusion after 20 min MCAO. The results are presented as mean ± SD of normalized expression of target gene with respect to GAPDH mRNA from three sets of animals. (C) Treatment with BW-B 70C prevented the nuclear translocation of p65 subunit of NF-κB (i-iii); the vehicle-treated animals showed nuclear translocation (ii) and treatment with BW-B 70C reversed this (iii) at 24 h reperfusion after 20 min MCAO. Figures are representative of similar results obtained from three groups of animals. A (i-iii) magnification 100 X; C (i-iii) magnification 200 X. *p < 0.01 vs. vehicle (n = 3).