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Table 2 Primary antibodies used for immunostaining and western blot

From: Production of IL-16 correlates with CD4+ Th1 inflammation and phosphorylation of axonal cytoskeleton in multiple sclerosis lesions

Antigen Clone Dilution Form Source Immuno/WB
IL-16 14.1 1:1000   BD Biosciences San Diego CA WB
IL-16 14.1 1:200 PE BD Biosciences San Diego CA IC
G155-178 (control) 1:200 PE BD Biosciences San Diego CA IC
Caspase-3 Polycloal 1:1000   R&D Systems Minneapolis MN WB
Active- Caspase-3 Polycloal 1:200   R&D Systems Minneapolis MN IC
T-bet Poly6235 1:500   Biolegend San Diego CA WB
Stat-1 Polycloal 1:1000   Cell Signaling Technology Co Danvers MA WB
Stat-6 Polycloal 1:1000   Cell Signaling Technology Co Danvers MA WB
NF(H+M) NP1 1:500   Chemicon Temecula CA WB
GAPDH Polycloal 1:1000   Santa Cruz Biotechnology Santa Cruz CA WB
CD3 APA1/1 1:100 FITC BD Biosciences San Diego CA IC
CD4 RPA-T4 1:100 FITC BD Biosciences San Diego CA IC
CD8 3b5 1:100 FITC Caltag Laboratories Bulingame CA IC
CD11b/Mac-1 ICRF44 1:50 Biotin BD Biosciences San Diego CA IC
CD20 H147 1:500 FITC Caltag Laboratories Bulingame CA IC
CD83 HB15e 1:500 FITC Caltag Laboratories Bulingame CA IC