Table 2 Primary antibodies used for immunostaining and western blot
Antigen | Clone | Dilution | Form | Source | Immuno/WB |
|---|---|---|---|---|---|
IL-16 | 14.1 | 1:1000 | Â | BD Biosciences San Diego CA | WB |
IL-16 | 14.1 | 1:200 | PE | BD Biosciences San Diego CA | IC |
G155-178 | (control) | 1:200 | PE | BD Biosciences San Diego CA | IC |
Caspase-3 | Polycloal | 1:1000 | Â | R&D Systems Minneapolis MN | WB |
Active- Caspase-3 | Polycloal | 1:200 | Â | R&D Systems Minneapolis MN | IC |
T-bet | Poly6235 | 1:500 | Â | Biolegend San Diego CA | WB |
Stat-1 | Polycloal | 1:1000 | Â | Cell Signaling Technology Co Danvers MA | WB |
Stat-6 | Polycloal | 1:1000 | Â | Cell Signaling Technology Co Danvers MA | WB |
NF(H+M) | NP1 | 1:500 | Â | Chemicon Temecula CA | WB |
GAPDH | Polycloal | 1:1000 | Â | Santa Cruz Biotechnology Santa Cruz CA | WB |
CD3 | APA1/1 | 1:100 | FITC | BD Biosciences San Diego CA | IC |
CD4 | RPA-T4 | 1:100 | FITC | BD Biosciences San Diego CA | IC |
CD8 | 3b5 | 1:100 | FITC | Caltag Laboratories Bulingame CA | IC |
CD11b/Mac-1 | ICRF44 | 1:50 | Biotin | BD Biosciences San Diego CA | IC |
CD20 | H147 | 1:500 | FITC | Caltag Laboratories Bulingame CA | IC |
CD83 | HB15e | 1:500 | FITC | Caltag Laboratories Bulingame CA | IC |