Figure 6

Chondroitin sulfate proteoglycans-4 (CSPG-4) is induced by stab wounds, but not by IL-1β. A, Neocortices from 2 wild-type (WT) and 2 IL-1R1-null mice (KO) at 10 d after stab wound or protein samples from the contralateral cortex were analyzed by Western blot for CSPG-4. Each lane represents protein from an individual animal. B, Samples from injected neocortices were homogenized in chondroitinase ABC and analyzed by Western Blot for CSPG-4. Each lane represents an individual WT animal that received either IL-1β or PBS. C, IL-1β was injected into WT or IL-1R1-null mice. Neocortices from 4 WT and 4 IL-1R1-null mice at 5 d after injecting 1 ng IL-1β were analyzed by Western blot for CSPG-4. Each lane represents protein from an individual animal.