Figure 5

Dexamethasone blocks the activation of MMP-2 in HCSM cells treated with pathogenic Aβ. Near confluent cultures of HCSM cells were incubated for six days in the presence or absence of 25 μM Dutch mutant Aβ40 with or without daily administration of anti-inflammatory drug (1 μM final concentration). The cell culture medium was collected and analyzed by gelatin zymography. Two or three separate experiments were performed in triplicate for each tested condition. A representative zymogram is shown.