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Table 1 Cytokine and apoptosis-associated message levels in nontransgenic and G93A-SOD1 astrocyte cultures in the basal (unstimulated) condition and 4 hours following stimulation with 50 U/mL IFNγ, 20 ng/mL TNFα or a combination of both cytokines. Data represent pooled samples from 6 wells in a typical experiment (see Fig. 1). Band intensities were normalized to the sum of GAPDH + L32 message levels prior to comparison between genotypes.

From: Primary glia expressing the G93A-SOD1 mutation present a neuroinflammatory phenotype and provide a cellular system for studies of glial inflammation

mRNA Unstimulated G93A-SOD1 as % of unstimulated nonTg Stimulated message level as % of unstimulated level, within genotypes
   NonTg stimulated with: G93A-SOD1 stimulated with:
   IFNγ TNFα IFNγ + TNFα IFNγ TNFα IFNγ + TNFα
Caspase 8 237 108 98 108 181 177 233
FADD 168 90 114 126 106 114 100
FAF 94 94 87 70 101 100 92
FAP 129 136 103 151 149 128 220
FAS 145 186 622 757 868 777 1392
IL1α 329 136 252 103 100 203 110
IL1β 644 152 1740 1101 161 724 223
IL1-RA 147 157 114 159 180 163 373
IL6 34 148 768 991 1461 1228 2755
IL18 307 221 104 92 199 205 194
IL12p35 341 141 239 263 108 71 103
IL12p40 325 121 137 165 197 94 1402
IFNγ 158 121 480 798 534 605 1792
MIF 70 83 129 133 95 110 108
RIP 159 130 152 225 230 161 258
TNFα 428 700 512 1511 2302 1669 3352
TNF-RI 166 132 99 131 180 112 176
TGFβ1 353 99 122 145 94 116 115
TGFβ3 155 90 96 87 94 132 148
TRADD 182 111 101 125 125 105 115
TRAIL 284 300 458 749 1307 734 1644