Figure 2
The effect of Aβ1–40 peptide on microglial proliferation at 48 hours. Microglial cultures were incubated with 1 μM fibrillar (f) or soluble (s) Aβ1–40, the NADPH oxidase inhibitor apocynin (10 μM), and/or with the hydrogen peroxide converters catalase (60 IU/ml) and EUK-8/-134 (20 μM) for 48 hours. After a 48 hour incubation, microglial cells were counted and their numbers expressed as percentage of cell number at time '0'. The dashed line indicates cell number at time '0'. Data are expressed as mean of 5 experiments ± standard error. Statistical analysis used: Student's t-test, p < 0.05; * – significant difference compared to the control; # – significant difference compared to samples treated with fibrillar Aβ only.