Figure 5
The effect of NADPH oxidase inhibitor and hydrogen peroxide scavengers on 24 hour treatment with Aβ1–40 peptide-induced TNF-α release from microglia. Microglial cultures were incubated with 1 μM fibrillar (f) or soluble (s) Aβ1–40, and/or with 10 μM apocynin, 60 IU/ml catalase, or 20 μM EUK-8/-134 for 24 hours. Then, TNF-α concentrations were measured in the cell incubation media. DMEM – microglial incubation medium not pre-incubated with cells. Data are expressed as mean of 6 experiments ± standard error; statistical analysis used: Student's t-test, p < 0.05; * – significant difference compared to the control; # – significant difference compared to samples treated with fibrillar Aβ only.