Figure 8
The effect of anti-TNF-α antibody on fibrillar Aβ1–40-induced microglial proliferation. Microglial cultures were incubated with 1 μM fibrillar (f) Aβ1–40, or fAβ together with either 40 μg/ml anti-rat TNF-α antibody or 10 ng/ml soluble TNF receptor inhibitor, or with 10 pg/ml TNF-α, alone or together with antibody or inhibitor, or with the antibody or the inhibitor alone for 24 hours. Cells were then counted and their numbers expressed as percentage of cell number at the start of the treatment, or time'0'. The dashed line indicates cell number at time '0'. sTNFRI – soluble TNF receptor inhibitor. Data are expressed as mean of 3–7 experiments ± standard error. Statistical analysis used: Student's t-test, p < 0.05; * – significant difference compared to the control; # – significant difference compared to samples treated with fibrillar Aβ1–40 only; & – significant difference compared to TNF-α only-treated samples.