Table 2 Primary antibodies used for immunohistochemistry.
Antibody | Target | Manufacture | Working dilution |
|---|---|---|---|
BrdU | 5-bromo-2'-deoxy-uridine | Becton Dickinson Cat. no. 347580 | 1:200 |
COX-2 | Cyclooxygenase 2 enzyme | Cayman Chemical Company Cat. no. 160126 | 1:4000 |
ED-1 | Glycoprotein of 90–100 kD expressed on the lysomal membrane of activated microglia, macrophages and monocytes. | Chemicon International Cat. no. MAB1435 | 1:4000 |
NeuN | DNA binding neuron-specific protein | Chemicon International Cat. no. MAB377B | 1:1000 |