Table 3 Pre-treatment with IFN-γ increases prostaglandin E2 production in response to amyloid-β or HuPrP82-146. SH-SY5Y neuroblastoma cells were pre-treated for 24 hours with 1 ng/ml cytokines as shown prior to the addition of amyloid-β or PrP peptides. Cells were lysed 24 hours later and total prostaglandin E2 levels were measured. Each value given represents the mean ± SD from triplicate experiments repeated twice (6 observations). ** = Prostglandin E2 levels significantly higher than untreated neurons incubated with amyloid-β1-42 (p < 0.05).
From: Interferon-γ increases neuronal death in response to amyloid-β1-42
Prostaglandin E2 (pg/ml) | ||||
|---|---|---|---|---|
Amyloid-β 1-42 | Amyloid-β 42-1 | HuPrP82-146 | HuPrP82-146sc | |
Control medium | 241 ± 62 | < 50 | 187 ± 40 | < 50 |
IFN-γ | 443 ± 112** | 66 ± 38 | 303 ± 55** | < 50 |
IL-1β | 228 ± 54 | < 50 | 202 ± 46 | < 50 |
IL-6 | 218 ± 66 | < 50 | 210 ± 48 | 55 ± 35 |
TNFα | 255 ± 82 | < 50 | 214 ± 33 | < 50 |