Figure 2
From: Interleukin-1β and anaphylatoxins exert a synergistic effect on NGF expression by astrocytes
Expression of NGF mRNA after stimulation of T98G celsl by MAP-C3a ( A ) or MAP-C5a ( B ) (10-8M). RNA was extracted after 2 h, 4 h, 6 h, 12 h and 24 h of stimulation and analyzed by semi-quantitative RT-PCR using GAPDH as internal standard. Bars represent the mean ± SEM for three independent experiments. **, p < 0.01, statistically significant compared with control as determined by Student's t test (NS = non stimulated cells).