Figure 3
From: Interleukin-1β and anaphylatoxins exert a synergistic effect on NGF expression by astrocytes
Expression of NGF mRNA after stimulation of T98G cell line by C3a or C5a anaphylatoxins (10-8M)- Effect of pre-incubation with anti-C3a or anti-C5aR antibodies. C3a anaphylatoxin was pre-incubated for 30 min with an anti-C3a antibody (diluted 1/100); or cells were pre-incubated for 30 min with an anti-C5aR antibody (diluted 1/100) before a 4 h stimulation by C3a or C5a (10-8M). Total RNA was extracted and RT-PCR was performed. Bars represent the mean ± SEM for three independent experiments. *, p < 0.05, statistically significant difference compared with non-stimulated cells (NS) as determined by Student's t test; #, p < 0.05, statistically significant difference compared with cells stimulated with C5a as determined by Student's t test.