Figure 7
From: Interleukin-1β and anaphylatoxins exert a synergistic effect on NGF expression by astrocytes
Effect of anaphylatoxins/IL-1β co-stimulation on NGF release. First, cells were stimulated for 48 h by a range of IL-1β concentrations to determine the maximal concentration of IL-1β that did not induce NGF release by T98G cells (A). Second, T98G cells were co-stimulated for 48 h with sub-optimal dose of IL-1β (0.5 U/ml) as well as with C3a, C5a, MAP-C3a or MAP-C5a (10-8M) (B). NGF release by rat astrocytes following MAP-C3a (10-8M) and IL-1β (0.5 U/ml) co-stimulation for 48 h is shown in (C). The concentration of NGF was measured in the supernatants by ELISA and was expressed as pg/ml per 106 cells. Bars represent the mean ± SEM for three independent experiments. +, p < 0.05, statistically significant compared with cells stimulated with anaphylatoxins or MAP-peptides alone as determined by Student's t test.