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Figure 3 | Journal of Neuroinflammation

Figure 3

From: All-trans retinoic acid induces COX-2 and prostaglandin E2 synthesis in SH-SY5Y human neuroblastoma cells: involvement of retinoic acid receptors and extracellular-regulated kinase 1/2

Figure 3

ATRA-induced COX-2 protein expression and PGE 2 production are inhibited by RAR pan-antagonist LE540 or MEK-1 inhibitor PD98059. (a) Western blot analysis of the expression of COX-2 protein in SH-SY5Y human neuroblastoma cells pre-incubated for 1 h with either RAR pan-antagonist LE540 2.5 μM (left) or 50 μM MEK-1 inhibitor PD98059 (right) and then incubated with ATRA (10 μM, 24 h). Equal protein loading was confirmed by probing with an anti-α-actin antibody. Normalized density ratio of COX-2 over α-actin is indicated for each band. Each experiment represents at least three repeated experiments. (b) PGE2 production in SH-SY5Y human neuroblastoma cells incubated for 24 hours with ATRA 10 μM in cells and pre-treated for 1 hour with either RAR pan-antagonist LE540 2.5 μM or 50 μM MEK-1 inhibitor PD98059. PGE2 in the medium was determined in triplicate in four separate experiments (for statistical purposes n = 4) (*P < 0.01 vs other groups)

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Journal of Neuroinflammation

ISSN: 1742-2094