Figure 2From: Effects of low dose GM-CSF on microglial inflammatory profiles to diverse pathogen-associated molecular patterns (PAMPs)Exposure of mixed glial cultures to low GM-CSF results in the ramification of resting microglia with a DC-like appearance. Primary microglia expanded either with or without GM-CSF were seeded onto 35-mm dishes at 2 × 106 cells per dish and incubated overnight in 6-well plates. The following day, cells were either unstimulated or treated with heat-inactivated S. aureus (107 cfu/well) for 24 h, whereupon bright field phase-contrast images were collected (40×). The results pictured are representative of two independent experiments.Back to article page