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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Effects of low dose GM-CSF on microglial inflammatory profiles to diverse pathogen-associated molecular patterns (PAMPs)

Figure 5

Phagocytic activity of primary microglia is not affected by GM-CSF. Primary microglia expanded either with (+) or without (-) GM-CSF were seeded onto 12 mm coverslips at 2 × 105 cells per coverslip and incubated overnight in 24-well plates. The following day, cells were treated with 2 × 106 heat-inactivated S. aureus-GFP (green) for 3 h and visualization of intracellular bacteria was detected using fluorescence microscopy (40×). Hoechst dye (blue) was used to visualize nuclei (A). In (B), the phagocytic index was calculated as the percentage of microglia which engulfed bacteria in a total of ten, 40 × microscopic fields. The results represent the mean ± SEM of two independent experiments.

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