Figure 1

HSV infection induces apoptosis in murine microglial cells. Wild-type and TLR2^-/- C57BL/6 microglial cells were infected with HSV at a MOI of 2. (A) The cells were examined for apoptotic DNA fragmentation using an oligonucleosomal ELISA at 8 and 24 h p.i. Data are presented as optical density (OD) per 10⁴ cells and are representative of three independent experiments using cells isolated from different brain specimens. (B) TUNEL staining of wild-type and TLR2^-/- microglia at 24 h p.i. After fixing and staining the wells, TUNEL positive cells from at least five fields were counted for each well. Data presented were representative of three independent experiments.