Effects of in vitro hypoxia on MCP-5 expression in mouse HIF-1α+/- and HIF-1α+/+ astrocytes. The cells were exposed to a 6 h in vitro hypoxia. MCP-5 mRNA expression and immunoreactive protein secretion were determined by RT-PCR (A) and ELISA (B), respectively, as described in Materials and methods. Each bar represents the mean ± SD of relative density/volumes of the bands on film negatives from at least three experiments or three ELISA assays. Asterisks and number signs indicate significant difference compared to relevant controls (p < 0.01; one-way ANOVA, followed by multiple comparisons among means).