Regulation of intracerebral expression of Bcl-2 (A), Fas (B), and C1-Inh (C) genes after head injury, as assessed by semi-quantitative two-step real-time RT-PCR. Total RNA was extracted from homogenized murine brains at 4 hours, 24 hours, and 7 days after traumatic brain injury (TBI). The murine primers for GAPDH, Bcl-2, Fas and C1-Inh are depicted in table 1. The technique for real-time RT-PCR analysis is described in the methods section. See text for details. Data are shown as means ± SD of n = 3 in the "nil" group and n = 6 per time-point in all other groups. *P < 0.05 for TBI_mAb 1379 vs. TBI_placebo groups; #P < 0.05 for TBI vs. sham groups; **P < 0.05 for TBI_placebo vs. TBI_mAb 1379 vs. placebo group (unpaired Student's t-test).