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Table 1 Murine primer sequences used for real-time RT-PCR analysis of intracerebral gene expression

From: Inhibition of the alternative complement activation pathway in traumatic brain injury by a monoclonal anti-factor B antibody: a randomized placebo-controlled study in mice

 

Gene ID at NCBI *

GeneBank Accession No.

Length of amplicons

Primer sequence

Probe Sequence

Order No. Qiagen

GAPDH #

14433

NM_008084

136 bp

commercially available Genexpression Assay QuantiTect Mm_GAPD

241012

Bcl-2

12043

NM_009741

NM_177410

118 bp

commercially available Genexpression Assay QuantiTect Mm_Bcl-2

241118

Fas

14102

NM_007987

96 bp

commercially available Genexpression Assay QuantiTect Mm_ Tnfsf6

241122

C1-Inh

12258

NM_009776

134 bp

AACTTAGAACTCATCAACACCT

GTTATCTTCCACTTGGCACTC

ACACCTGCCTCGTCCT

custom made

  1. * NCBI, National Center for Biotechnology Information
  2. # Housekeeping gene