Histopathological features of MOG-EAE during the acute and remission stages. Spinal cord sections from a rat in the acute stage (day 13 post immunization) of EAE show extensive inflammation involving the white and grey matter (D), with marked demyelination in the inflammatory areas (F). The majority of the infiltrating inflammatory cells are macrophages, as evidenced by positive staining for the ED-1 marker (E). During the remission phase (day 18 post immunization), inflammation (G) and infiltration of macrophages (H), as well as demyelination (I) are substantially reduced. A normal control rat is included for comparison (A, B, C). H&E staining (A, D, G), ED-1 immunohistochemistry (B, E, H), LFB/PAS staining (C, F, I). Magnification: lens × 4.