Figure 1
From: 15d-PGJ2 induces apoptosis of mouse oligodendrocyte precursor cells
The effect of PGs on the growth and survival of mouse oligodendrocyte precursor (mOP) cells. (A) mOP cells express the oligodendrocyte precursor surface markers NG2 (red) and A2B5 (Green). Scale bar, 20 μm. (B) mOP cells were treated with PGE2 or PGF2α (0.1, 1 and 10 μM) and examined using the MTT assay after 24 h. (C-D) mOP cells were treated with the indicated concentrations of PGD2 or 15d-PGJ2 and examined using the MTT assay after 24 h. Data are the average of 3–4 experiments and expressed as percentage of the control group (vehicle treated). (E) mOP cells were treated with 15d-PGJ2 (1 μM) and examined using the LDH assay after 24 h and 48 h. Data are expressed as percentage of the total LDH. Asterisks indicate significant difference versus control group (One-way ANOVA with Dunnet posttest for C and D, Student t-test for E, *(p < 0.05), **(p < 0.01).