Figure 2

Characterization of 15d-PGJ2-induced apoptosis in mOP cells. mOP cells were treated with 15d-PGJ2 (1 μM) for 24 h, and then stained with the nuclear dye bisbenzimide or labeled with the TUNEL method. Cells with condensed nuclei (A) or that were TUNEL-positive (B) were counted, and expressed as percentage of the total number of cells. For apoptosis ELISA (C) and DNA fragmentation gel analysis (D), DNA from mOP cells that had been treated with 15d-PGJ2 (5 μM) for 24 h was extracted and analyzed. Staurosporine (STA, 100 nM) treatment was used as positive control. M, molecular standards. Asterisks indicate significant difference versus control group (t-test, two-tailed, *(p < 0.05), **(p < 0.01)).