Figure 3

15d-PGJ2 cytotoxicity on mOP cells occurs independently of PPARγ or PGD2 membrane receptors. mOP cells cultured on coverslips were treated with 15d-PGJ2 (1 μM), in the absence or presence of the irreversible PPARγ antagonists GW9662 (GW, 10 μM) or T0070907 (T, 100 nM), or nonspecific PGD2 receptor (DP) antagonist AH6809 (AH, 10 μM) or specific DP2 antagonist BAY-u3405 (BAY, 5 μM) for 24 h, and apoptotic cell death was examined using bisbenzimide staining. Cells were counted and expressed as percentage of control (untreated). Data shown were average of three experiments. Asterisks indicate significant difference versus control group (Two-way ANOVA Bonferroni posttest, **(p < 0.01), ***(p < 0.001)).