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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Prostaglandin E2 receptor subtype 2 (EP2) regulates microglial activation and associated neurotoxicity induced by aggregated α-synuclein

Figure 4

Lack of EP2 reduced translocation of p47-phox but not p67-phox subunit from cytoplasm to membrane in microglia after α-synuclein oligomer treatment. WT or EP2-/- microglia were seeded in 6-well plates overnight and then treated with aged α-synuclein or vehicle for 30 min. The cells were homogenized and fractionated into cytoplasm (cyt) and membrane fractions (mem). The relative distribution of p67-phox (panel A) and p47-phox (panel B) in the two fractions was analyzed with Western blot in order to assess translocation from the cytoplasm to the membrane (expressed as ratio of relative amount in membrane fraction to membrane + cytoplasm). *: p < 0.05, comparing EP2-/- with WT microglia. **: P < 0.01, comparing α-synuclein treatment with control.

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