From: Microglial cells in astroglial cultures: a cautionary note
Ref. | Species-Age-Region | [LPS] μg/ml | Co-activators | Time post-LPS | Presence in astrocytes* | Presence in microglia (marker) | Notes | Double labelling method** |
---|---|---|---|---|---|---|---|---|
[29] Fig 4 | Mouse-Neonatal-Whole Brain | 1 | - | - | Yes (?) | Yes (Mac1) | "The number of GFAP/NOS2 positive cells was low". NOS2(+) cells have microglial morphology and not a clear GFAP staining | DAB-Ni and DAB |
[68] Fig 2 | Rat-Neonatal-Cortex | 100 | - | 24 h | No | - | NOS2(+) cells have a microglial appearance but no double labelling is done | IF |
[69] Fig 1 | Rat-Neonatal-Cortex | 1 | - | 24 h | No | Yes (Isolectin-B4) | Â | IF |
[70] Fig 1 | Rat-Neonatal-Forebrain | 1 | - | 12 h, 24 h, 48 h | No | - | NOS2(+) cells have a microglial appearance but no double labelling NOS2/microglia is done | DAB and AP |
[71] Fig 3 | Rat-Neonatal-Cortex | 2 | IFNγ (100 U/ml) | 18 h | No | Yes (OX-42) |  | IF |
[72] Fig 2 | Rat-Neonatal-Cortex | 2 | IFNγ (100 U/ml) | 18 h | No | Yes (OX-42) |  | IF |
[73] Fig 3 | Rat-Neonatal-Cortex | 2 | IFNγ (100 U/ml) | 18 h | - | Yes (OX-42) | "All NOS2(+) cells were OX-42(+)" | IF |
[74] Fig 2 | Rat-Neonatal-Cortex | 2 | IFNγ (100 U/ml) | 18 h | No | - |  | IF |
[39] Figs 2,3,5 | Rat-Neonatal-Cortex | 10 | - | 48 h | No | Yes (OX-42) | Â | IF |
[30] Fig 3 | Rat-Neonatal-Neopallium | 0,025 | IFNγ (100 U/ml) | 24 h | Yes (?) | - | Some double GFAP-NOS2(+) cells do not look like astrocytes | IF |
[75] Fig 5 | Rat-Neonatal-Cortex | 1 | IFNg (100 U/ml) | 48 h | No | Yes (Isolectin-B4) | Â | IF |
[28] Fig 1 | Rat-Neonatal-Spinal cord | 1 | - | 24 h | Yes | - | Double GFAP-NOS2(+) cells. Double NOS2-microglial staining not done because "these cultures had no OX42 positive cells" | IF |
[76] Fig 3 | Rat-Neonatal-Cortex | 0,1 | - | 24 h | No | Yes (OX-42) | "All NOS2(+) cells were OX-42(+)" | IF |
[27] Fig 2 | Rat-Neonatal-Cortex | 1 | IFNγ (10 ng/ml) | 14 h | Yes | Yes (Isolectin-B4) | "NOS2 staining is much stronger in microglia than in astrocytes" | IF |
[24] Fig 6 | Mouse-Neonatal-Cortex | 1 | CGS21680 (100 nM) | 48 h | No | Yes (Tomato lectin) | Â | IF |
[26] Fig 6 | Mouse-Neonatal-Cortex | 2 | IFNγ (3 ng/ml) | 24 h | Yes | - | Double GFAP-NOS2(+) cells | IF |
[25] Fig 8 | Mouse-Neonatal-Cortex | 2 | IFNγ (3 ng/ml) and TGFβ1 (3 ng/ml) | 14 h | Yes | - | Double GFAP-NOS2(+) cells. The number of NOS2(+) astrocytes is very low after LPS but high after TGFβ1 + LPS + IFNγ treatment | IF |