Figure 6

S-Mtb-induced microglial activation is not associated with TLR2 or dectin-1. A) Measurement of TNF-α and IL-6 from cultures stimulated with 1 μg/ml LPS, 1% s-Mtb, or 19-kDa (0.5 or 1 μg/ml). Latex beads, heated Mtb, and H37Ra were added with MOI at 1. The supernatants were harvested at 18 h and cytokine production was quantified by ELISA. Data are presented as the percentage of the control and compared to cultures incubated with LPS. B) Comparison of cytokine production in mixed glial cells from WT and TLR2 -/- mice. Primary mixed glial cells from WT and TLR2-/- mice were prepared and stimulated with 1% s-Mtb, 1 μg/ml LPS, 10 μg/ml PGN, or 10 nM BLP. The supernatants were harvested at 6 h and 18 h and analyzed for TNF-α, IL-6, and IL-12p40 production by ELISA. Data are presented as the percentage of the control and compared to cultures incubated with s-Mtb for 6 h. C) BV-2-cells were stimulated with 1% s-Mtb in the presence of PBS or a neutralizing monoclonal antibody against dectin-1. After 1 h of incubation at 37°C, the supernatants were analyzed for cytokine production by ELISA. Data are presented as the percentage of the control and compared to cultures incubated with s-Mtb alone. IC, isotype control.