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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Developmental stage of oligodendrocytes determines their response to activated microglia in vitro

Figure 4

OPC proliferation as measured by BrdU incorporation. To assure that reduced OPC survival in the presence of LPS-activated microglia was not due to a reduction in OPC proliferation, a BrdU incorporation study was conducted. Some OPC nuclei (A) also incorporated BrdU (B). OPCs were also labeled with antibody to A2B5 (C) to confirm that BrdU positive cells were OPCs. OPC proliferation, as assessed by cell counts, was not altered by microglia or LPS-activated microglia (though there was a trend toward increased OPC proliferation in the presence of microglia, p = 0.056, factorial ANOVA for main effect of microglia) verifying that lower OPC numbers were due to OPC loss rather than reduced OPC proliferation (D, error bars = SEM).

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