Figure 1
From: Amyloid precursor protein modulates β-catenin degradation
APP expression induced degradation of β-catenin. A. Overexpression of APP reduces β-catenin levels. Human APP695 or the control LacZ was expressed in primary neurons via HSV vectors at 1 or 2 IU per cell during 14 h of infection. The control HSV vector carries the β-galactosidase gene (LacZ). B. Quantitative densitometric analyses of western blots from 4 independent experiments. An average 44% reduction in normalized mean optical density (representing protein levels) was observed for 2 IU/cell of APP compared to the corresponding LacZ control (p < 0.05, t-Test). Shorter exposures were used for densitometry analyses; longer exposures also showed the expression of endogenous APP, but densitometric analyses were not possible due to the solidified chemiluminescence reaction products.