Figure 5

β-Catenin and cyclin D1 are significantly elevated in hippocampal CA1 pyramidal cells in APP knockout mice compared to those in WT controls. Brain sections from APP knockout and WT mice (n = 4 each) were co-labelled with rabbit anti-β-catenin and mouse anti-cyclin D1 together with Alexa Fluor 594 and Alexa Fluor 488 secondary antibodies. The sections were also counter-stained with DAPI to visualize the cell body layer. Images were captured at the same settings with a Nikon Eclipse 600 microscope. Representative images from an APP knockout and a WT animal are presented in A. The pixel density of specific staining was randomly sampled in the CA1 cell body layer and the mean values from each group of animals are shown in the bar graphs: B. β-catenin, two-tail t-Test, APP knockout vs AT, p = 0.001; C. cyclin D1, two-tail t-Test, APP knockout vs WT, p = 0.02.