Skip to main content
Figure 1 | Journal of Neuroinflammation

Figure 1

From: Differential effects of Th1, monocyte/macrophage and Th2 cytokine mixtures on early gene expression for glial and neural-related molecules in central nervous system mixed glial cell cultures: neurotrophins, growth factors and structural proteins

Figure 1

Effect of Th1 cytokines on microglia. CNS mixed glial cultures were incubated with Th1 cytokines or additional culture medium for 4 days. Cultures were fixed with 4% paraformaldehyde for 10 minutes, washed and then incubated with mouse anti-ED-1 (IgM) and mouse anti-rat MHC class II (IgG) followed by Alexa 488-conjugated goat anti-mouse IgM and Cy 3-conjugated donkey anti-mouse IgG. Cultures were examined for indirect immunofluoresence employing a Leitz Orthoplan 2 fluorescent microscope. The Th1 treated microglia (ED-1+ cells) have a different appearance when compared to those incubated with additional medium (control). Control microglia do not express MHC class II whereas Th1 treated microglia strongly express MHC class II molecules.

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094