Figure 5

Aβ plaques were not detected but tyrosine hydroxylase positive neurons were present in op/op mice. The rabbit polyclonal anti-Aβ antibody, DW14, did not detect Aβ deposition in WT (A) or op/op mice (B) in the hippocampus or overlying cortex of 30 day-old mice. A cryosection from a 12 month-old APP-tg mouse (C) was included in the same immunohistochemistry experiment as a positive control and shows abundant Aβ plaques. Tyrosine hydroxylase (TH) was used to immunolabel dopaminergic neurons in the substantia nigra. There were comparable numbers of TH expressing neurons in WT (D) and op/op (E) mice. The morphology of the dopaminergic neurons was similar in both groups of 30 day-old mice. An adjacent section was incubated with normal rabbit IgG as a negative control (F) for the TH immunostaining and shows no labeled cells. CA1- field CA1 of hippocampus, CC- corpus callosum, DG- dentate gyrus, SNC- substantia nigra, compact. Scale bar A-C – 250 μm Scale bar D-F – 100 μm.