Effects of 10 daily doses of ethanol exposure on LPS-induced liver, serum and brain IL-1βproduction. Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for 10 days and injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Liver, serum and brain samples were collected 1 hr after LPS injection. LPS significantly increased IL-1βprotein in liver, serum and brain after 10 days of ethanol administration compared with LPS alone treatment. The results are the means ± SEM of two experiments performed with 6 mice each group. * P < 0.05, ** P < 0.01, compared with the saline control mice. # P < 0.05, ## P < 0.01, compared with LPS-treated mice.